Development of Simple and Rapid Diagnostic Method for Strawberry Latent Ring-spot Virus in Plants Using Loop-Mediated Isothermal Amplification Assay

Strawberry Latent Ring-spot Virus (SLRSV) was first discovered in Scotland and classified in the genus Nepovirus as a plant pathogen of the Group IV positive-sense ssRNA viruses. SLRSV is classified intothe family Secoviridae (International Committee on Taxonomy of Viruses; ICTV)and is transmitted by seeds and soilinhabiting nematodes (Xiphinemadiversicaudatum and X. coxi). The infection by SLRSV forms tubule-like structures, facilitating the mechanism of cell-cell translocation through plasmodesmata. Some of the typical symptoms after infection include chromatic and/ or morphological alteration of leaves. 

This virus has a wide host range, attacking many economically cultivated crops. For example, SLRSV was recently found to infect olive in Syria, Oriental hybrid lily in Northern India, strawberry fields in United States, and black locust (Robinia pseudacacia L, family Fabaceae) in Poland. The hosts of this virus in Korea are Rosa spp., Aesculusspp., Trifolium spp., Peteroselinum crispum, Vitis spp., Fritillaria imperialis, Humulus lulus, Euonymus europaeus, Pastinica sativa, Laminum amplexicaule, Ribes spp., Rubus spp., Delphinum spp., Fragaria ananassa, Muscari spp., Lilium spp., Paunus spp., Apium graveolens, Narcissus spp., Robinia peudoacacia, Asparagus densiflorus, Prunus avium, and Prunus.