Endorhizospheric bacteria also known as endophytic bacteria
that resides within living plant tissues without substantially harming plants
have found a large number of applications in today’s agriculture such as
nutrient cycling, tolerance to biotic and abiotic stress as well as promotion
ofplant growth. In present study an attempt has been made to test biodiversity ofendophytic plant growth promoting bacteria from different plant species. As
nitrogen fixation is one of the prime mechanism of plant growth promoting
activity exerted by microorganisms, endophytic nitrogen fixing bacteria were
isolated from from surface sterilized plant parts viz. root, stem and leaves of
species Cynodondactylon (Durva), Pothosscandens (Money plant), Ipomeabatata
(Sweet potato), Saccharumofficinarum (Sugarcane) cv. CO.LK-8001 and CO.-84135,
Musa paradica (Banana) and Zea mays (maize)cv. GM-6 by using nitrogen free
media selective for growth of nitrogen fixing bacteria. In all total 10
isolates were obtained from different plant species and parts and showed
variation in their biochemical and physiological characteristics. All the
isolates were found to fix nitrogen and nitrogen fixation rates of all the
isolates ranged from 4.0 to 36.3 mg N fixed/gm of sugar consumed. Moreover,
they also showed solubilizing tri calcium phosphate in Pikovskaya’s broth.
It is well known that a large number of microorganisms are
found to be associated with plants. These plant associated microorganisms arethe agents for stimulation of plant growth and management of plant and soilhealth. They colonize roots and rhizosphere i.e. the soil surface closely
adhered to the plant roots. Microorganisms on the roots and in the rhizosphere
get benefits from root exudates.
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